Based on the stability test results on each anti-aging serum formulas, formula with E. This stability test observed physical properties pH, viscosity, refractive index, and specific gravity and chemical properties. Algae crude extract was formulated into anti-aging serum formulation and evaluated for one month by accelerated stability test method. Algae extract in this study also proven to have low toxicity with LC 50 higher than 1000 ppm. oligocystum is stated in IC 50 value which are 2693.33, 2679.16, 4407. µg/ml respectively wherein it still considered as low antioxidant activity. australis has the highest total phenol and flavonoid content that is 106 mgGAE/100 gr and 30.516 mgQE/g respectively. Padina Australis, Ulva Reticulata, Sargassum Oligocystum, dan Euchema Cottonii contain chemical constituent hexadecanoic acid, methyl ester, fatty acid and phytol which possess antioxidant activity. The purpose of this study was to investigate the chemical constituent, total phenol and flavonoid content, antioxidant activity and toxicity of algae extract and formulated into serum preparation. Red, brown and Green algae contain antioxidant and have the potential as anti-aging but still rarely developed for cosmetic ingredients.
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